On-the-Spot Gene Readouts Offer Clues to How Cells Work

On-the-Spot Gene Readouts Offer Clues to How Cells Work

The new method combines a technology called tissue expansion with an in situ sequencing approach. With the information inside cells now easier to see, the next step involves a technique called FISSEQ (fluorescent in situ sequencing), which generates readouts of thousands of mRNA molecules in cells [3]. Or, it can compile a more-comprehensive readout of gene activity within cells by analyzing as many gene readouts as detectable. They discovered, for example, that immune B cells near tumor cells expressed certain inflammatory genes at a higher level than immune B cells that were further away. Links:Ribonucleic Acid (RNA) (National Human Genome Research Institute/NIH)Synthetic Neurobiology Group (Massachusetts Institute of Technology, Cambridge)George Church (Harvard Medical School, Boston)NIH Support: National Human Genome Research Institute; National Cancer Institute; National Institute of Biomedical Imaging and Bioengineering; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke

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